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KMID : 1007520030120050533
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Food Science and Biotechnology
2003 Volume.12 No. 5 p.533 ~ p.539
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Immunomodulating Activity of Pectic Polysaccharides from Eleutherococcus senticosus
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Yoon, Taek Joon
Lee, Sang-Hoon/Hwang, Soo Hyun/Shin, Kwang-Soon/Park, Woo-Mun/Hwang, Jong-Hyun/Yu, Kwang-Won
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Abstract
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To screen the immunomodulating activity of Elrutherococcus senticosus, E. senticous was extracted by cold-water, hexane, MeOH, and hot-water, consecutively. The hot-water extract (EN-0) had the most potent bone marrow cell proliferating activity through Peyer´s patch. EN4 was fractionated into a MeOH-soluble fraction (EN-1), EtOH-soluble fraction (EN-2), and crude plysaccharide fraction (EN-3). Among these fractions, only EN-3 had the most potent immunomodulating activities, and contained about 25.9% galacturonic acid with arabinose. glucose, galactose, and xylose (molar ratio; 0.57: 1.00:0.50:0.65) as the major neutral sugars, and a small quantity of protein (4.7%). In treatments of EN-3 with NaIO₄ and pronase, pronase digestion did not significantly affect the immunological activities of EN-3, but periodate oxidation reduced the bone marrow cell proliferating activity (63.0% at 100 ㎍/mL) as well as the tumor metastasis inhibiting activity of EN-3 (68.8% at 50 ㎍/mouse). In addition, EN-3 was further fractionated by anion-exchange chromatography on DEAE-Sepharose CL-6B into eight subfractions (EN-31 and EN-3IIa-3IIg). EN-3IIe was mainly composed of arabinose, glucose, galactose, xylose, and galacturonic acid. and showed moderate immunomodulating activities. EN-3IIc and 3Ild consisted of arabinose, galactose, glucose, xylose, and galacturonic acid (molar ratio; 0.31:0.34:0.60:0.36:1.00 and 0.20:0.22:0.36:0.21:1.00) as the component sugars with a small quantity of proteins (3.5 and 5.5%), and EN-3IId had more potent immunological activities than EN-3IIc based on these results. It may be concluded that bone marrow cell proliferating and tumor metastasis inhibiting activities of EN-3IId are caused by pectic polysaccharides containing a polygalacturonan moiety with neutral sugars, such as glucose and xylose.
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